Ramphastidae (Aves: Piciformes) are animals recognized for their exuberant colours and long bill, with distribution range from south Mexico to north Argentina. They are important seed dispersers eating little vertebrates eventually. When in captivity, animals usually live in limited spaces, with high density and near by species that do not share a evolution history, facilitating the occurrence of infectious diseases for what they may not have a competent immune system against, including the parasitic ones. This study analyzed the endoparasites that occur in captive Ramphastidae at São Paulo Zoo in the period January 2009 to September 2011. Seven species of toucans and toucanets had parasitological results positive for Trichuridae nematodes, Eimeria sp., Giardia sp., non sporulated coccidian oocists and unidentified nematode eggs, and microfilarie was the only hemoparasite found.