The increase of contact between natural and rural areas is prominent in Brazil, due to agricultural activities and concern with the environmental conservation. In this context, domestic animals, wild fauna and humans are exposed to mutual exchange of parasites, microorganisms and diseases. We studied tick parasitism of wild carnivores and domestic dogs, and the environmental of questing ticks, in extensive cattle ranch areas intermingled with natural vegetation, and in a natural reserve, both in a region of Cerrado biome, Midwestern Brazil. From 2008 to 2015, we inspected 119 wild carnivores from nine species, and collected six tick species (Amblyomma sculptum, Amblyomma ovale, Amblyomma dubitatum, Amblyomma tigrinum, Dermacentor nitens and Rhipicephalus microplus). The most numerous and infested hosts were Cerdocyon thous, Lycalopex vetulus, Chrysocyon brachyurus, Puma concolor and Conepatus amazonicus. From 139 domestic dogs, we collected A. sculptum, Rhipicephalus sanguineus and R. microplus. From vegetation, samplings resulted in A. sculptum, A. dubitatum, A. ovale, Amblyomma rotundatum and R. microplus, with dominance of A. sculptum. Domestics and wild animals presented high overlapping of infestations by A. sculptum, a generalist and anthropophilic tick species. This tick is the most important vector of the Brazilian spotted fever, a lethal human disease. This fact elicits attention and requires efforts to monitor the presence of pathogens vectored by ticks circulating in this type of agroecosystem, including in other regions of the Brazil, because the most of the natural vegetation remaining have been increasingly immersed in pastures and agricultural matrix.
Trypanosoma cruzi and Leishmania spp. are parasites that infect multiple hosts including canids, considered bioaccumulators of parasites. Deforestation in the Cerrado biome has resulted in the exposure of wild canids to anthropized areas, where they may establish ecological and epidemiological relationships with domestic dogs. We evaluated the infection by trypanosomatids in canids from a Cerrado agroecosystem between 2013 and 2017. Samples of wild canids (blood, bone marrow and skin) and dogs (blood) were collected for parasitological, serological and molecular diagnosis. A total of 414 samples from wild (n = 131) and domestic (n = 283) canids were collected, including recaptures. We obtained five positive hemocultures from Lycalopex vetulus (n = 2), Cerdocyon thous (n = 1) and dogs (n = 2), all characterized as T. cruzi TcIII/V (18S rDNA) and TcIII/V/VI (gGAPDH); one positive skin fragment for Leishmania sp. (C. thous), one positive skin culture (Chrysocyon brachyurus) and one positive fresh blood examination from a dog. Infection by T. cruzi and Leishmania spp. was serologically confirmed in 18% and 4% of the canids, respectively. Active transmission was attested by seroconversion events and occurred despite the low rate of positive parasitological assays. Wild and domestic canids infected by both parasites were detected sharing the same areas, pointing to a possible spillover of parasites among them.