Objective: describe wild fauna casuistry data evaluated in 2019, in a reference laboratory in the State of São Paulo, Brazil, through histopathological and immunohistochemical analysis after the implementation of a pilot project of wildlife disease laboratory surveillance. Methods:descriptive study with survey of samples of non-human primates, birds and other mammals, received from two screening centers in the city of São Paulo. For this purpose, the referral forms and the issued histological and immunohistochemical reports were reviewed and data was tabulated and analysed by frequency and percentage distribution. Results: 233 animals of 20 distinct genera and/or species were received, being 191 (81,9%) non-human primates, 25 birds (10,7%) and 17 other mammals (7,3%); zoonotic pathogens were detected among the studied population, with a prevalence of bacterial conditions among the conclusive cases. Conclusion:histological and immunohistochemical analysis of wild fauna samples due the implementation of a wildlife disease laboratory surveillance pilot program contributed to the detection of some pathogens of public health relevance in São Paulo city, Brazil.