Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomicand metatranscriptomic-based analyses of a large composting operation in the Sao Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50°C to 75°C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process; and that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomassdegrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology.

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Categories: Artigo
Author: Aline Maria da Silva, Andrew Maltez Thomas, Deibs Barbosa, George Willian Condomitti Epamino, Gianluca Major Machado Silva, João Batista da Cruz, João Carlos Setubal, Julio Cezar Franco de Oliveira, Karen Cristina Lombardi, Layla Farage Martins, Leandro Nascimento Lemos, Livia Maria Silva Moura, Luciana Principal Antunes, Luciano Antonio Digiampietri, Patricia Locosque Ramos, Renata Castiglioni Pascon, Roberta Verciano Pereira, Ronaldo Bento Quaggio